MODERN ANALYTICAL TECHNIQUES (MPY 101) SYLLABUS

MODERN ANALYTICAL TECHNIQUES (MPY 101) SYLLABUS

Theory

1. Theory, Instrumentation, Methods and Applications of VU Spectrophotometer.

2. Theory and Instrumentation of IR and FT-IR, its advantage and applications in Structural elucidation.

3. NMR, C13 NMR, Origin of spectra, Chemical shifts, Spin-spin coupling,
Coupling constant, Instrumentation and application for Structural elucidation.

4. Mass spectra, Instrumentation, Fragmentation pattern and applications
for Structural elucidation. Application of GC-Mass, HPLC-Mass for complex mixtures.

5. Theory, Instrumentation and application for the following:
i) Fluorescence
ii) X – Ray crystallography
iii) Atomic spectroscopy
iv) Ultra centrifugation
v) ESR
vi) Liquid Scintillation spectrometry
vii) Auto radio grapy

6. Separation Techniques; Fundamental principles, Basic instrumentation, Qualitative and Quantitative Pharmaceutical applications of Gas-liquid Chromatography, HPLC, HPTLC, 
Gel Chromatography, Electrophoresis and Ion-pair Chromatography.

7. General Principle, instrumentation and application of optical 
rotatory dispersion (ORD) and Circular dichroism (CD).

8. Immunoassay Techniques: Enzyme and Radioimmunoassay
techniques. Theory, Methods and applications.

9. Thermal methods: Thermo Gravimetry (TG), Differential Scanning 
Calorimetry (DSC), Differential Thermal Analysis (DTA).

10. Principles and application of light, Phase contrast, Scanning and
Transmission electron microscopy, Cytometry and Flow cytometry.

Books and References Recommended:

1. Florey, Analytical Profiles of Drugs, Vol.1-16.
2. Sinder, Text Book of HPLC.
3. McLafferty, Mass Spectrometry.
4. Rao,C.N., Ultraviolet Visible Spectroscopy for Chemical Application.
5. Silverstein, Basseler, Morril, Spectophotometrc Identification of Organic
Compounds.
6. Rao,C.N., Chemical Application of Infrared Spectroscopy.
7. Weissberger, Physical Methods in Organic Chemistry.
8. Kiencz, B. and Dierasi, C., Interpretation of Mass Spectra of Organic
Compounds.
9. Jackmann, Application of NMR Spectra to Organic Compounds.
10.Willard, Merrit and Dean, Instrumental Methods of Analysis.
11. Elliel, E.L., Stereochemistry of Carbon Compounds.
12. Naahod, P., Physical Methods of Structure Determination.
13. Stahl, Thin Layer Chromatography.
14. Ewing, Instrumental Methods of Chemical Analysis.
15. Block and Durrum, Paper Chromatography and Electrophoresis.
16. Remington’s Pharmaceutical Sciences.
17. Sirmer, Spectroscopic Analysis.